Specifications

Applications- SDS-PAGE, Mass Spectrometry, etc.

Sample Type- Protein Lysate

Sample Volume- 2uL

Kit Size- 20 Assays, 96 well plate Assay

Product Details

ITSI’s Total Protein Assay Kit (ToPA) comes in two formats one for use with cuvettes and another for use with 96 well plates. Both kits include all the reagents necessary for the assay as well as a premade standard curve with an r2 of greater than 99 and a full set of cuvettes or a 96 well plate depending on the format purchased

Principle

ToPA is a simple ready to use kit based on the proven Bradford Assay. ToPA has been optimized to be more sensitive than the standard Bradford assay while also being more tolerant to standard concentrations of common buffer systems. With our ready to use reagents and validated procedure you can generate consistent reproducible results with as little as 2ul of sample in under 5 minutes per reading.

Procedure

Using the included Standard Curve Reagents a standard curve is prepared.  A small amount of sample is then combined with our Quanti-Protein Assay Reagent (Q-PAR) and the absorbance is measured. The resulting absorbance is then plotted against the standard curve to determine the protein concentration of the sample.

Applications

Protein concentration measurement is commonly the first step for many proteomics processes. ToPA simplifies the process and allows you to move on to the next step of your workflow quickly with consistent reproducible results.

Supporting Data and Figures

Each Kit contains optimized reagents and consumables including:

• Quanti-Protein Assay Reagent
• Ready-to-Use Standard Curve Reagents
• 2000 ug/ml SCR
• Cuvettes or microtitre plates
• 1.5ml Assay Tubes
• Validated lab procedures