Human Serum Albumin (HSA, 66 kDa) typically accounts for 65% or more of the total protein present in serum and plasma samples. This can prevent the detection of low abundant plasma and serum proteins in most experiments. Albumin also distorts lanes during electrophoresis if a large amount of total protein is loaded to permit the detection of low abundant proteins. Albumin can also mask other proteins that migrate around 50-70 kDa thereby preventing their detection. Benefits of using ITSIPrep Albumin Removal Kits:

• Lowest cost/sample
• Ready-to-use reagents
• Reproducible results
• Depletes Greater than 80% albumin
• Easy-to-follow protocol

ITSIPrep ASKs (K-0013) is a validated and cost effective organic solvent-based protocol for selective depletion of albumin (Alb) from serum and plasma samples and analysis of the albumin-enriched fraction (Alb+) and albumin depleted (Alb-) fractions. More than 85% of albumin is depleted from whole plasma using the ITSIPrep ASKs Kit and Protocol (see Figure). This protocol has been tested and successfully used to improve the chances of revealing lesser abundant proteins in the Alb- fraction or albumin-associated proteins in the Alb+ fraction. Treated samples can be analyzed by electrophoresis (1D-PAGE, 2D-PAGE, 2D-DIGE), mass spectrometry and western analysis. Each Kit contains optimized buffer systems and consumables including:

• ASKs Buffer 1
• ASKs Buffer 2
• ASKs Buffer 3
• ProPreCip Precipitation Booster
• Microgrinder pestles and tubes

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